Donald J. Johann, Jr., M.S., M.D.


Associate Professor of Medicine and Biomedical Informatics
Director of Informatics

M.D., Case Western Reserve University, Cleveland, Ohio

Fellowship, Medical Oncology, National Cancer Institute, National Institutes of Health, Bethesda, MD

Fellowship, Hematology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD

Postdoctoral Fellowship, Clinical Proteomics, National Cancer Institute, National Institutes of Health, Bethesda, MD, under the mentorship of Lance Liotta, MD, PhD.


American Society of Clinical Oncology, American Society for Mass Spectrometry, American Association for Cancer Research

Research Interests:

Application of advanced molecular profiling and high-throughput technologies for the characterization of molecular alterations in cancer cells. Areas of emphasis include high-resolution identity-based mass spectrometry, laser capture microdissection (LCM), bioinformatics, and cancer biology.

Selected Publications:

Mukherjee S, Rodriquez-Canales J, Hanson J, Emmert-Buck MR, Tangrea MA,  Prieto DA, Blonder J, Johann D.  Proteomic Analysis of Frozen Tissue Samples Using Laser Capture Microdissection.  2012 Methods in Molecular Biology.  2012  (accepted for publication).

Ye X, Luke BT, Johann DJ, Chan CC, Prieto DA, Ono A, Veentra TD, Blonder J.  Post-digestion 18O exchange/labeling for quantitative shotgun proteomics of membrane proteins.  Methods in Molecular Biology. 2012 893:223-240.

Wei BH, Simpson RM, Johann DJ, Dwyer J, Prieto DA, Ye X, Luke B, Shive HR, Webster JD, Hoover SB, Veenstra TD, Blonder J.  Proteomic profiling of H-Ras-G12V induced hypertrophic cardiomyopathy in transgenic mice using comparative LC-MS analysis of thin fresh-frozen tissue sections. J Proteome Res. 2012  Mar 2;11(3):1561-70.

Johann DJ, Mukherjee S, Prieto DA, Veenstra TD, Blonder J.   Profiling solid tumor heterogeneity by LCM and biological MS of fresh frozen tissue sections. Methods in Molecular Biology.  2011 755:95-106.

Ye X, Luke BT, Johann DJ,  Ono A, Prieto DR, Chan KC, Issaq HJ, Veenstra TD, Blonder J.  Optimized method for computing 18O/16O ratios of differentially stable-isotope labeled peptides in the context of post digestion 18O exchange/labeling.  Analytical Chemistry. 2010 July 82(13): 5878-86.

Johann DJ, Bih-Rong W, Prieto DA, Chan KC, Ye X, Rudnick PA, Xiao Z, Stein SE, Issaq HJ, Veenstra TD, Blonder J.  Combined Blood/Tissue Analysis for Cancer Biomarker Discovery.  Analytical Chemistry.  2010 March 1;82(5): 1584-8.

Ye X, Johann DJ Jr, Hakami RM, Xiao Z, Meng Z, Ulrich RG, Issaq HJ, Veenstra TD, Blonder J. Optimization of protein solubilization for the analysis of the CD14 human monocyte membrane proteome using LC-MS/MS.  J Proteomics. 2009 Nov 2;73(1): 112-22.

Johann DJ, Rodriguez-Canales J, Mukherjee S, Prieto DA, Hanson JC, Emmert-Buck M, Blonder J.  Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry.  J Proteome Res. 2009 May; 8(5):2310-8.

Mbeunkui F, Johann DJ Jr. Cancer and the tumor microenvironment: a review of an essential relationship.  Cancer Chemother Pharmacol. 2009 Mar;63(4):571-82.

Blonder J, Johann DJ, Veenstra TD, Xiao Z, Emmert-Buck MR, Ziegler RG, Rodriguez-Canales J, Hanson JA, Xu X.  Quantitation of steroid hormones in thin fresh frozen tissue sections. Analytical Chem. 2008 Nov 15;80(22):8845-52.

Johann DJ, Blonder J.  “Fluids vs. Tissues vs. Both”, Expert Reviews in Molecular Diagnostics. 2007.  Sept; 7(5):473-5.

Johann DJ Jr, Veenstra TD.  “Multiple Biomarkers in Molecular Oncology”, Expert Reviews in Molecular Diagnostics.   2007.  May; 7(3):223-5.

Petricoin E, Espina V, Araujo R, Midura B, Yeung C, Wan X, Eichler G, Johann D, Meyer W, Qualman S, Anderson J, Steinberg S, Tsokos M, Kartik K, Helman L, Liotta L.  “Phosphoprotein pathway mapping: AKT/mammalian target of rapamycin activation is negatively associated with childhood Rhabdomyosarcoma Survival”, Cancer Research. 2007 Apr 1;67(7):3431-40

Menard C, Johann D, Lowenthal M, Muanza T, Sproull M, Ross S, Gulley J, Liotta L, Coleman N, Petricoin E, Camphausen K.  “Discovering Clinical Biomarkers of Ionizing Radiation Exposure with Serum Proteomic Analysis”, Cancer Research, 2006; 66: (3)  1844 – 1850.

Johann D, McGuigan M, Patel A, Tomov S, Ross S, Conrads T, Veenstra T, Fishman D, Whiteley G, Petricoin E, Liotta L.  “Clinical Proteomics and Biomarker Discovery”, Annals of the New York Academy of Sciences, 2004: (1022) 295-305.